On Saturday, February 1, 2003, Space Shuttle Columbia disintegrated as it reentered the atmosphere over Texas and Louisiana, killing all seven astronauts on board. It was the second Space Shuttle mission to end in disaster, after the loss of Challenger and crew in 1986.

The mission, designated STS-107, was the twenty-eighth flight for the orbiter, the 113th flight of the Space Shuttle fleet and the 88th after the Challenger disaster. It was dedicated to research in various fields, mainly on board the SpaceHab module inside the shuttle's payload bay. During launch, a piece of the insulating foam broke off from the Space Shuttle external tank and struck the thermal protection system tiles on the orbiter's left wing. Similar foam shedding had occurred during previous Space Shuttle launches, causing damage that ranged from minor to near-catastrophic, but some engineers suspected that the damage to Columbia was more serious. Before reentry, NASA managers had limited the investigation, reasoning that the crew could not have fixed the problem if it had been confirmed. When Columbia reentered the atmosphere of Earth, the damage allowed hot atmospheric gases to penetrate the heat shield and destroy the internal wing structure, which caused the orbiter to become unstable and break apart.

After the disaster, Space Shuttle flight operations were suspended for more than two years, as they had been after the Challenger disaster. Construction of the International Space Station (ISS) was paused until flights resumed in July 2005 with STS-114.